智財技轉處 Department of Intellectual Property and Technology Transfer 智財技轉處 Department of Intellectual Property and Technology Transfer

本院覽號：28A-960213

創作人：Chi-Huey Wong, Tsui-Ling Hsu, Masaaki Sawa, Sarah R Hanson

智財權：US 7910319 B2

摘要：

The invention provides a method of labeling of cellular glycans bearing azide group via a fluorescent labeling technique. The method entails generating fluorescent probe form a nonfluorescent precursor, 4-ethynyl-N-ethyl-1,8-naphthalimide, by Cu(I)-catalyzed [3+2] cycloaddition (click activation) of alkyne group of the probe with an azido-containing fucose analog into glycoprotein via the fucose salvage pathway. Cells treated with 6-azidofucose are labeled with the click activated fluorogenic probe or biotinylated alkyne.

可能的應用範圍：

1. Glycoprotein imaging
2. Glycoprotein purification
3. Identification of key biomarkers
4. Cancer Research
5. Diagnosis of Disease

此項發明的優點：

Powerful technique for visualizing the intracellular localization of fucosylated glycoconjuates by fluorescent microscopy

智財技轉處聯絡人：

對本技術有興趣，請於本處網頁廠商選項下(廠商需求與諮詢)網頁填寫資料，承辦人將跟您聯絡。

If you are interested in this technology, please fill in the information on the webpage under the manufacturer option (Apply for Technology Licensing, Material Transfer, or Sponsored Research) on the website of this office, and the undertaker will contact you