創作人：Chi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng
智財權：US 8680020 B2、US 10274488 B2
The invention relates to a novel method to immobilize glycans onto aluminum coated glass (ACG) slides for potential use in disease diagnosis and drug discovery.
The quality of sugar chips can be assessed by mass spectrometry and fluorescence measurement with high sensitivity. To demonstrate this, we have synthesized a model compound of mannose with a built-in photoclevable linker bound to the ACG slide surface. The molecular weight of the grafted mannose was identified by mass spectroscopy. The slide was subjected bind biotinylated ConA binding, followed by Cy3-tagged streptavidin detectoin. We have observed that the immobilized mannose gave higher fluorescence signal intensity than that on glass slides in protein binding assay. In addition, there is a proportional relationship between the peak intensity of the mass of grafted sugar and the fluorescence intensity of the Cy3-tagged sugar binding protein.
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