This invention is an integrated system that performs fast antimicrobial susceptibility test to screen antibiotics in a few hours instead of days by conventional methods. This novel screening system will save lives of bacteremia patients who are in critical care by timely identifying the correct antibiotics to fight pathogens. By eradicating pathogens in time, it will even improve the odds of septicemia patients to recover from the septic shock, particularly when sepsis drugs are yet to be discovered. This system can also identify susceptible antibiotics to treat mycobacterial infection in a few days instead of the usual six to eight weeks. The concepts, methodologies, and devices to achieve fast antimicrobial susceptibility testing are identified in this invention. Fast screening of antibiotics is achieved by a short period of specimen incubation in different antibiotics embedded media to create differential bacterial counts. The differences of bacterial counts among antibiotics embedded media are subsequently amplified by DNA amplification methods for detection. Following DNA amplification, rapid quantification and minimum inhibition concentration (MIC) determinations for a panel of antibiotics are achieved in less than one minute by fluorescence quantification methods described in this invention.
The method allows rapid anti-microbial susceptibility testing for bacteria of indigenous flora, mycobacteria, fungi, and other bacterial pathogens. The technology can be applied in clinical diagnostics labs for critically ill patients who require immediate action in identifying effective antimicrobial agents.
The method allows rapid anti-microbial susceptibility testing to be completed in hours instead of days or weeks by the conventional methods. The invention also teaches a way to rapidly verify large number of PCR amplifications which are usually done by time consuming gel electrophoresis.
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