智財技轉處 Department of Intellectual Property and Technology Transfer 智財技轉處 Department of Intellectual Property and Technology Transfer

圖1. Generation of anti-PAC antibody and validation of its performance. The workflow of detecting S-nitrosylated proteins by anti-Cys-A antibody (up); Anti-phenylacetymidyl-cysteine (anti-PAC) antibody was generated using BSA as a carrier protein (down).

圖2. The PAC-switch method to probe S-nitrosylated proteins. Free thiols are blocked by sequential addition of IAM (-SX) and APIAM (-SY). S-nitrosylated Cys is reduced by ascorbate, tagged by IAN (-SZ), and then detected by anti-PAC antibody.