智財技轉處 Department of Intellectual Property and Technology Transfer 智財技轉處 Department of Intellectual Property and Technology Transfer

本院覽號：28A-980602

創作人：Kuo-Ping Chiu

智財權：US Pending

摘要：

Multiplex barcoded Paired-End Ditag (mbPED) library construction for ultra high throughput sequencing is disclosed. The mbPED library comprises multiple types of barcoded Paired-End Ditag (bPED) nucleic acid fragment constructs, each of which comprises a unique barcoded adaptor, a first tag, and a second tag linked to the first tag via the barcoded adaptor. The two tags are the 5′- and 3′-ends of a nucleic acid molecule from which they originate. The barcoded adaptor comprises a barcode, a first polynucleotide sequence comprising a first restriction enzyme (RE) recognition site, and a second polynucleotide sequence comprising a second RE recognition site and covalently linked to the first polynucleotide sequence via the barcode. The two REs lead to cleavage of a nucleic acid at a defined distance from their recognition sites. The length of the adaptor is set so that the bPED nucleic acid fragment fits one-step sequencing.

可能的應用範圍：

Worldwide DNA sequencing and proteomics markets are expected to rise to $17.5 billion in 2009. The novel sequencing approach is advantagous in the aspect of ultra high throughput sequencing, therefore it would have great market potentials.

此項發明的優點：

This is a sweet, creative design for making paired-end diTag library with barcode inserted in the adaptor position in the fragment library. The clone of “barcoded paired-end ditags (bPETs)” is constructed prior to libribary construction, thus the cloning process is quite flexible. Using the barcode as recognition signature, multiplex paired-end diTag (mPET) library could be created by mixing multiple bPET library together. In the end, ultra high throughput sequencing can be achieved with reduced sequencing running time and cost. Compared to current sequencing technology, the advantages of this novel sequencing approach include:

1. to simplify experimental procedure for PE-mediated investigations
2. to reduce sequencing running cost
3. to reduce sequencing time
4. to increase sequencing throughtput

智財技轉處聯絡人：

對本技術有興趣，請於本處網頁廠商選項下(廠商需求與諮詢)網頁填寫資料，承辦人將跟您聯絡。

If you are interested in this technology, please fill in the information on the webpage under the manufacturer option (Apply for Technology Licensing, Material Transfer, or Sponsored Research) on the website of this office, and the undertaker will contact you